Limitations of Sulforhodamine 101 for Brain Imaging
نویسندگان
چکیده
منابع مشابه
Limitations of Sulforhodamine 101 for Brain Imaging
Since 2004, the red fluorescent dye Sulforhodamine 101 (SR101) has been boosting the functional analysis of astrocytes in a functional environment in an unprecedented way. However, two major limitations have been challenging the usefulness of this tool for cellular imaging: (i) SR101 is not as specific for astrocytes as previously reported; and (ii) discoveries of severe excitatory side effects...
متن کاملActive Sulforhodamine 101 Uptake into Hippocampal Astrocytes
Sulforhodamine 101 (SR101) is widely used as a marker of astrocytes. In this study we investigated labeling of astrocytes by SR101 in acute slices from the ventrolateral medulla and the hippocampus of transgenic mice expressing EGFP under the control of the astrocyte-specific human GFAP promoter. While SR101 efficiently and specifically labeled EGFP-expressing astrocytes in hippocampus, we foun...
متن کاملStrong coupling between surface plasmon polaritons and Sulforhodamine 101 dye
We demonstrate a strong coupling between surface plasmon polaritons and Sulforhodamine 101 dye molecules. Dispersion curves for surface plasmon polaritons on samples with a thin layer of silver covered with Sulforhodamine 101 molecules embedded in SU-8 polymer are obtained experimentally by reflectometry measurements and compared to the dispersion of samples without molecules. Clear Rabi splitt...
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Over the several decades of its existence, brain imaging has moved from the laboratory to the bedside. Brain imaging now plays a routine role in the diagnosis of many central nervous system disorders. However, the appeal of a “snapshot” of the brain extends beyond medicine. Brain imaging, now available in color, with its simplicity and vividness, has sometimes proven irresistible to defense att...
متن کاملDevelopmental profile and properties of sulforhodamine 101--Labeled glial cells in acute brain slices of rat hippocampus.
The reliable identification of astrocytes for physiological measurements was always time-consuming and difficult. Recently, the fluorescent dye sulforhodamine 101 (SR101) was reported to label cortical glial cells in vivo [Nimmerjahn A, Kirchhoff F, Kerr JN, Helmchen F. Sulforhodamine 101 as a specific marker of astroglia in the neocortex in vivo. Nat Methods 2004;1:31-7]. We adapted this techn...
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ژورنال
عنوان ژورنال: Frontiers in Cellular Neuroscience
سال: 2017
ISSN: 1662-5102
DOI: 10.3389/fncel.2017.00044